Title

PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria

Document Type

Article

Publication Date

12-1-2008

Abstract

We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the P(BAD) promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

Comments

This article was published in Appl Environ Microbiol. 2008 December; 74(23): 7422–7426 and published online 2008 October 10. doi: 10.1128/AEM.01369-08