Heme Induced Oxidative Stress Attenuates Sirtuin1 and Enhances Adipogenesis in Mesenchymal Stem Cells and Mouse Pre-Adipocytes

Document Type


Publication Date

Summer 6-2012


Patho-physiological conditions with high oxidative stress, such as conditions associated with increased denatured heme-proteins, are associated with enhanced adipogenic response. This effect predominantly manifests as adipocyte hypertrophy characterized by dysfunctional, proinflammatory adipocytes exhibiting reduced expression of anti-inflammatory hormone, adiponectin. To understand how increased levels of cellular heme, a pro-oxidant molecule, modulates adipogenesis; the following study was designed to evaluate effects of heme on adipogenesis in human mesenchymal stem cells (hMSCs) and mouse pre-adipocytes (3T3L1). Experiments were conducted in the absence and in the presence of a superoxide dismutase mimetic (tempol, 100μM). Heme (10μM) increased (p


The version of record is available from the publisher at http://dx.doi.org/10.1002%2Fjcb.24061. Copyright © 2012 Wiley Periodicals, Inc. All rights reserved.