BACKGROUND: The Western diet is high in omega-6 fatty acids and low in omega-3 fatty acids. Canola oil contains a healthier omega 3 to omega 6 ratio than corn oil. Jurkat T leukemia cells were treated with free fatty acids mixtures in ratios mimicking that found in commercially available canola oil (7% α-linolenic, 30% linoleic, 54% oleic) or corn oil (59% linoleic, 24% oleic) to determine the cell survival or cell death and changes in expression levels of inflammatory cytokines and receptors following oil treatment.
METHODS: Fatty acid uptake was assessed by gas chromatography. Cell survival and cell death were evaluated by cell cycle analyses, propidium-iodide staining, trypan blue exclusion and phosphatidylserine externalization. mRNA levels of inflammatory cytokines and receptors were assessed by RT-PCR.
RESULTS: There was a significant difference in the lipid profiles of the cells after treatment. Differential action of the oils on inflammatory molecules, following treatment at non-cytotoxic levels, indicated that canola oil mimetic was anti-inflammatory whereas corn oil mimetic was pro-inflammatory.
SIGNIFICANCE: These results indicate that use of canola oil in the diet instead of corn oil might be beneficial for diseases promoted by inflammation.
Ion G., Fazio K., Akinsete JA, Hardman WE. Effects of canola and corn oil mimetic on Jurkat cells. Lipids in Health and Disease 2011 10:90.
Biochemistry Commons, Biology Commons, Medical Biochemistry Commons, Medical Cell Biology Commons, Medical Microbiology Commons, Microbiology Commons, Other Biochemistry, Biophysics, and Structural Biology Commons