Here we report expression and characterization of recombinant bacterial phytase PaPhyC from Pantoea sp. Codon-optimized phytase gene was expressed E.coli BL21 pLysS and protein expression was confirmed by Western blotting. Recombinant protein expressed in E.coli has high phytase activity. We show that PaPhyC recombinant phytase has different molecular masses when expressed in bacteria and plants, suggesting that possible protein glycosylation in plants may influence its overall size.
Khabipova NN, Valeeva LR, Chastukhina IB, Sharipova MR, Shakirov EV. Heterologous Expression of Pantoea Agglomerans Phytase Gene Optimized for Plant-Host Expression. International Journal of Advanced Biotechnology and Research. 2016;7(2):683-888.