Date of Award


Degree Name



College of Science

Type of Degree


Document Type


First Advisor

Leslie Frost


Prohibitin, a highly conserved protein found in eukaryotic cells, has been found in recent years to possess a wide variety of functions in the cell. Whereas the mechanisms by which the protein functions are still largely unknown, it is clear that prohibitin possesses very strong anti-proliferative properties in the cell, as well as potentially both nuclear and mitochondrial functions based upon its movement and localization upon apoptotic signaling. Upon treatment with retinoic acid, transformed B16 mouse melanoma cells also indicate a differential expression of prohibitin in which production of the protein is increased. The purpose of this work was to both validate the initial findings reported using this cell line and 2D gel electrophoresis, as well as attempt to identify, isolate, and analyze prohibitin using mass spectrometry in order to determine if in its function within the cell, prohibitin was in any way being modified. Although several proteins such as carbamoyl phosphate synthase I, heat shock protein 70, and annexin A5 were identified as part of this experiment, prohibitin was never isolated by immunoprecipitation and was only observed using western blotting.


Tumor suppressor proteins.