Date of Award
College of Science
Type of Degree
Fatty acids influence immune responses, but how this occurs at the molecular level is not understood. Class I major histocompatibility complex molecules (MHC I), transmembrane proteins required for antigen presentation to T cell receptors, each consist of a 45kDa α chain noncovalently associated with a 12-kDa β2-microglobulin (β2m) molecule. Docosahexaenoic acid’s (DHA) effect on the binding of β2m and anti-MHC I monoclonal antibodies (Mab) was measured. EL4 and RMA-S cells were cultured 48 hours with 0-45µM DHA or oleic acid. Murine to human β2m exchange and Mab binding were assessed by fluorescence flow cytometry. DHA-treated cells showed increased fluorescence (greater β2m binding and Mab binding) compared to untreated and oleic acid-treated cells. The addition of α-tocopherol to these fatty acid treatments eliminated the DHA-associated increase in fluorescence. DHA’s effect on the cell membrane may alter the conformation of MHC I affecting antigen presentation to CD8+ T-lymphocytes and altering immune response.
Immune response - Effect of acid on.
Hypes, Kaleb Marie, "Docosahexaenoic Acid Modulates Class I Major Histocompatibility Complex Protein Function" (2004). Theses, Dissertations and Capstones. 660.