Heme Induced Oxidative Stress Attenuates Sirtuin1 and Enhances Adipogenesis in Mesenchymal Stem Cells and Mouse Pre-Adipocytes
Patho-physiological conditions with high oxidative stress, such as conditions associated with increased denatured heme-proteins, are associated with enhanced adipogenic response. This effect predominantly manifests as adipocyte hypertrophy characterized by dysfunctional, proinflammatory adipocytes exhibiting reduced expression of anti-inflammatory hormone, adiponectin. To understand how increased levels of cellular heme, a pro-oxidant molecule, modulates adipogenesis; the following study was designed to evaluate effects of heme on adipogenesis in human mesenchymal stem cells (hMSCs) and mouse pre-adipocytes (3T3L1). Experiments were conducted in the absence and in the presence of a superoxide dismutase mimetic (tempol, 100μM). Heme (10μM) increased (p
Puri, N., Sodhi, K., Haarstad, M., Kim, D.H., Bohinc, S., Foglio, E., Favero, G. and Abraham, N.G., 2012. Heme induced oxidative stress attenuates sirtuin1 and enhances adipogenesis in mesenchymal stem cells and mouse pre‐adipocytes. Journal of Cellular Biochemistry, 113(6):1926-1935.