Author

Anjaiah Katta

Date of Award

2007

Degree Name

Biology

College

College of Science

Type of Degree

M.S.

Document Type

Thesis

First Advisor

Eric R. Blough

Second Advisor

David Mallory

Third Advisor

Charles Somerville

Abstract

Increased muscle loading results in phosphorylation of the 70 kDa ribosomal S6 kinase (p70s6k) signaling pathway and this event strongly correlates with degree of muscle adaptation following resistance exercise. Here, we compared the basal and contraction- induced phosphorylation of p70s6k, Akt and mTOR in tibialis anterior muscles of lean and obese Zucker rats. Immunoblotting demonstrated differences in level of basal p70S6k phosphorylation (Thr 389) in the normal and diabetic TA. HFES had an increase in p70S6k (Thr389) phosphorylation at 0-, 1- and 3-hr in lean TA and only at 3-hr in obese TA. mTOR (Ser 2448) Phosphorylation was elevated in lean TA immediately after HFES and remains unaltered in obese TA. HFES increased activity of both Akt (Thr 308) and Akt (Ser 473) in lean TA. These results suggest that diabetes is associated with alterations in the muscle content and ability to activate p70s6k signaling following an acute bout of exercise.

Subject(s)

Non-insulin-dependent diabetes.

Musculoskeletal system.