Sarath Meduru

Date of Award

2007

Degree Name

Biology

College

College of Science

Type of Degree

M.S.

Document Type

Thesis

First Advisor

Eric R. Blough

Second Advisor

David Mallory

Third Advisor

Guo- Zhang Zhu

Abstract

We have previously reported that aging in the Fisher 344 X Brown Norway (FBN) rat aorta is characterized by increased levels of ROS and alterations in cell signaling. Acetaminophen was found to scavenge free radicals in recent ischemia-reperfusion studies. Here we examined if chronic treatment with a therapeutic dose of acetaminophen attenuates age-associated increase in aortic ROS accumulation and signaling. FBN rats (27 month old; n=8) were subjected to 6 months of treatment with a therapeutic dose of acetaminophen (30mg/kg/day), with age-matched untreated FBN rats as controls. Protein oxidation levels were altered in control and treated aortae compared to aortae from 6 month animals. Immunoblotting analysis revealed that activated levels of c- Jun-N-Terminal kinase (JNK), Erk1/2 and AMPK levels were altered with aging and treatment. Activated p38-MAPK levels were altered with aging. Our data suggest that chronic acetaminophen treatment alters age associated ROS signaling in FBN rat aorta.

Subject(s)

Aorta -- Aging.

Acetaminophen -- Toxicology.

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