Date of Award


Degree Name

Biomedical Sciences


Joan C. Edwards School of Medicine

Type of Degree


Document Type


First Advisor

Dr. Travis Salisbury, Committee Chairperson

Second Advisor

Dr. Richard Egleton

Third Advisor

Dr. Larry Grover


The mammalian target of Rapamycin (mTOR) is a Serine/threonine kinase that is found in two large protein complexes that are known as mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). Torin 1 is an ATP-competitive inhibitor of mTOR that inhibits mTOR activity in both mTORC1 and mTORC2. In this study, we hypothesized that dual inhibition of mTORC1 and mTORC2 with Torin 1 promotes the apoptosis of clear cell renal cell carcinoma cells. The human clear cell renal cell cancer line, 786-0, was treated with Torin 1, and markers of apoptosis, including PARP, Caspase 3, and Caspase 9, and their cleaved subunit proteins were studied using the Western Blot technique. To further investigate the role of mTORC2, short interfering RNA (siRNA) experiments in 786-0 cells were performed to selectively reduce the expression of Rictor, the defining subunit of mTORC2. Our findings demonstrate increased expression of Caspase 3 and Cleaved PARP in 786-0 cells treated with Torin1 which confirms activation of apoptosis. No human or animal was involved in this study.


Renal cell carcinoma – Immunological aspects – Research.

Available for download on Thursday, May 01, 2025