Date of Award
2023
Degree Name
Biomedical Sciences
College
Joan C. Edwards School of Medicine
Type of Degree
M.S.
Document Type
Thesis
First Advisor
Dr. Travis Salisbury, Committee Chairperson
Second Advisor
Dr. Richard Egleton
Third Advisor
Dr. Larry Grover
Abstract
The mammalian target of Rapamycin (mTOR) is a Serine/threonine kinase that is found in two large protein complexes that are known as mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). Torin 1 is an ATP-competitive inhibitor of mTOR that inhibits mTOR activity in both mTORC1 and mTORC2. In this study, we hypothesized that dual inhibition of mTORC1 and mTORC2 with Torin 1 promotes the apoptosis of clear cell renal cell carcinoma cells. The human clear cell renal cell cancer line, 786-0, was treated with Torin 1, and markers of apoptosis, including PARP, Caspase 3, and Caspase 9, and their cleaved subunit proteins were studied using the Western Blot technique. To further investigate the role of mTORC2, short interfering RNA (siRNA) experiments in 786-0 cells were performed to selectively reduce the expression of Rictor, the defining subunit of mTORC2. Our findings demonstrate increased expression of Caspase 3 and Cleaved PARP in 786-0 cells treated with Torin1 which confirms activation of apoptosis. No human or animal was involved in this study.
Subject(s)
Renal cell carcinoma – Immunological aspects – Research.
Recommended Citation
Farimand, Samaneh, "Inhibition of the mTOR signaling pathway in clear cell renal cell carcinoma cells" (2023). Theses, Dissertations and Capstones. 1769.
https://mds.marshall.edu/etd/1769
