Date of Award


Degree Name

Exercise Science


College of Education

Type of Degree


Document Type


First Advisor

Matthew Comeau

Second Advisor

William Marley

Third Advisor

Terry Shepherd


It is common for athletes to use blood lactate for the determination of performance as well as for the development of training volumes. Depending on the mode, duration, and intensity, the sample site at which blood lactate is drawn becomes important. Due to the debate of sample site, research has been ongoing. There has yet to be a collective investigation examining three sample sites and maximal anaerobic exercise while considering the role of inactive muscle. The purpose of this study was to gain better understanding of blood lactate concentrations at various sample sites after maximal effort exercise. Eight anaerobically trained males completed a 30 second Wingate test. Blood samples were collected after resting supine for 30 minutes before the test, directly after the test, and again 10 minutes post. All three analyses were significant (p < 0.05) with the sample site X sample time interaction, sample time main effect, and sample site main effect being reported, respectively: Wilks’ Λ = 0.004, F (4,4) = 274.33, p = 0.000, Wilks’ Λ = 0.039, F (2,6) = 74.111, p = 0.000, and Wilks’ Λ = 0..213, F (2,6) = 11.066, p = 0.010. Post-hoc paired t-tests on computed mean differences between samples sites for each sample time revealed statistically significant differences (p < 0.05) between several time intervals. Further analysis also revealed significant differences (p < 0.05) in the sample site main effect between the toe and finger. To avoid misinterpretation caused by the pooling of lactate in non-exercising muscles, blood samples should be collected from a site closer to the active muscles.


Blood lactate


Exercise - Physiological aspects


Physical fitness - Testing