A new species of Ananteris Thorell, 1891 is herein described from a single locality placed in the Cordillera Central of Colombia, which represents the first record of this genus for Tolima department. This new taxon exhibits several singular features, and apparently represents a relict population inside an urbanized area. Also, some aspects on the taxonomy of the genus are briefly discussed, and its diagnosis is emended. With this addition, the total of Ananteris species described from Colombia is raised to six.