The results of the study of new samples of scorpions from Colombia are presented. Tityus erikae Lourenço, 1999 is demonstrated to be a junior synonym of Tityus tayrona Lourenço, 1991, and the adult female of Tarsoporosus macuira Teruel et Roncallo, 2007 is described for the first time. Also, new locality records and supplementary information on morphological variability (including some diagnosis updates) are given for Centruroides margaritatus (Gervais, 1841), Rhopalurus caribensis Teruel et Roncallo, 2008, Tityus tayrona, and Tarsoporosus macuira.