Diplocentrus izabal sp. n. is herein described on basis to one male (holotype) and two females from El Estor municipality, Izabal department, eastern Guatemala. It seems to be a close relative of Diplocentrus lachua Armas, Trujillo & Agreda, 2012, from which it differs by having carapace almost smooth, with anteromedian notch V-shaped; pectines with 11 teeth in both sexes, and carapace clearly longer than pedipalp manus and metasomal segment V (D. lachua has carapace minutely granulate, with anteromedian notch U-shaped; pectines with 13 teeth in the males (female unknown), and carapace as long as both pedipalp manus and metasomal segment V).