Document Type

Article

Publication Date

Fall 8-6-2012

Abstract

Maneuverable, high aspect ratio poly 3-4 ethylene dioxythiophene (PEDOT) fibers are fabricated for use as cellular force probes that can interface with individual pseudopod adhesive contact sites without forming unintentional secondary contacts to the cell. The straight fibers have lengths between 5 and 40 μm and spring constants in the 0.07-23.2 nN μm-1 range. The spring constants of these fibers were measured directly using an atomic force microscope (AFM). These AFM measurements corroborate determinations based on the transverse vibrational resonance frequencies of the fibers, which is a more convenient method. These fibers are employed to characterize the time dependent forces exerted at adhesive contacts between apical pseudopods of highly migratory D. discoideum cells and the PEDOT fibers, finding an average terminal force of 3.1 ± 2.7 nN and lifetime of 23.4 ± 18.5 s to be associated with these contacts.

Comments

This is the authors’ peer-reviewed final manuscript submission. The version of record is available from the publisher at http://iopscience.iop.org/0957-4484/23/45/455105/. Copyright: © 2012 IOP Publishing Ltd. All rights reserved.

doi:10.1088/0957-4484/23/45/455105

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