Title
PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria
Document Type
Article
Publication Date
12-1-2008
Abstract
We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the P(BAD) promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.
Recommended Citation
Qiu, Dongru; Damron, F. Heath; Mima, Takehiko; Schweizer, Herbert P.; and Yu, Hongwei D., "PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria" (2008). Biochemistry and Microbiology. 10.
https://mds.marshall.edu/sm_bm/10
Comments
This article was published in Appl Environ Microbiol. 2008 December; 74(23): 7422–7426 and published online 2008 October 10. doi: 10.1128/AEM.01369-08