Ouabain-Stimulated Trafficking Regulation of the Na/K-ATPase and NHE3 in Renal Proximal Tubule Cells

Authors

Yanling Yan, Marshall UniversityFollow
Steven Haller
Anna Shapiro
Nathan Malhotra
Jiang Tian
Zijian Xie
Deepak Malhotra
Joseph I. Shapiro MD, Marshall UniversityFollow
Jiang Liu, Marshall UniversityFollow

Document Type

Article

Publication Date

Spring 5-23-2012

Abstract

We have demonstrated that ouabain regulates protein trafficking of the Na/K-ATPase a1 subunit and NHE3 (Na/H exchanger, isoform 3) via ouabain-activated Na/K-ATPase signaling in porcine LLC-PK1 cells. To investigate whether this mechanism is species-specific, ouabain-induced regulation of the a1 subunit and NHE3 as well as transcellular 22Na? transport were compared in three renal proximal tubular cell lines (human HK-2, porcine LLC-PK1, and AAC-19 originated from LLC-PK1 in which the pig a1 was replaced by ouabain-resistant rat a1). Ouabain-induced inhibition of transcellular 22Na? transport is due to an ouabain-induced redistribution of the a1 subunit and NHE3. In LLC-PK1 cells, ouabain also inhibited the endocytic recycling of internalized NHE3, but has no significant effect on recycling of endocytosed a1 subunit. These data indicated that the ouabain-induced redistribution of the a1 subunit and NHE3 is not a species-specific phenomenon, and ouabain-activated Na/K-ATPase signaling influences NHE3 regulation.

Comments

The final publication is available at http://link.springer.com/article/10.1007/s11010-012-1331-x#page-1. Copyright © 2012 Springer Science+Business Media. All rights reserved. DOI 10.1007/s11010-012-1331-x

Recommended Citation

Yan, Y., Haller, S., Shapiro, A., Malhotra, N., Tian, J., Xie, Z., ... & Liu, J. (2012). Ouabain-stimulated trafficking regulation of the Na/K-ATPase and NHE3 in renal proximal tubule cells. Molecular and Cellular Biochemistry, 367, 175-183.