Date of Award
2017
Degree Name
Biological Sciences
College
College of Science
Type of Degree
M.S.
Document Type
Thesis
First Advisor
Mary Yeager-Armstead
Second Advisor
Gary Schultz
Third Advisor
Elmer Price
Fourth Advisor
Leslie Frost
Abstract
Prymnesium parvum (P. parvum) is a toxin producing haptophyte that has spread globally over the past 100 years. The algae, initially a marine organism, has moved inland to less saline waters. In addition, there is variability in the efficacy of the toxins produced from bloom to bloom. Variation between strains has been suggested as the reason for bloom differences; however, environmental variability within a single strain must first be established. Here, a single strain of P. parvum was cultured at three environmentally relevant salinities, 6psu, 13psu, and 35psu. Three different environmental shocks, decreased temperature, raised pH, and addition of specific ions were applied to cultures of alga at each of the three salinities. Supernatant from each condition was used to test variability in hemolytic, cytolytic, and ichthyiotoxic properties. Culture salinity influenced growth rate of the algae, with increased growth rates at higher, marine salinities. Ichthyotoxicity of toxins produced were also increased with increased culture salinity. Cytolytic and hemolytic properties were found to vary with culture salinity; however, these properties were greatest at a culture salinity of 13psu. Environmental shocks also caused significant variability in efficacy of toxins produced. These tests demonstrate variability in toxic effect within a single strain of P. parvum and provide insight into the wide array of toxic effect in blooms worldwide.
Subject(s)
Algal blooms -- Research.
Hypoxia (Water) -- Research.
Toxic algae -- Research.
Prymnesium parvum -- Research.
Recommended Citation
Parsons-White, Amy, "Establishing environmental variability in the toxins produced by a single strain of prymnesium parvum" (2017). Theses, Dissertations and Capstones. 1134.
https://mds.marshall.edu/etd/1134
Included in
Biology Commons, Ecology and Evolutionary Biology Commons, Environmental Health Commons, Toxicology Commons