Isolation and Identification of Hsc70 Conjugates in RAW264.7 Murine Macrophage-Like Cells

Author

Michael Arland ParsonsFollow

Date of Award

2019

Degree Name

Chemistry

College

College of Science

Type of Degree

M.S.

Document Type

Thesis

First Advisor

Dr. John Rakus, Committee Chairperson

Second Advisor

Dr. Menashi Cohenford

Third Advisor

Dr. Leslie Frost

Abstract

IL12R- β2 is a Type I cytokine receptor and contains the WXXW (WSNWS) sequence that often predicates the post-translational addition of mannose to a tryptophan residue via a carbon-carbon bond. This study will stimulate expression of IL12R- β2 in RAW 264.7 Macrophage-Like Cells by shutting down the extracellular signal kinase (ERK) pathway and introducing inflammatory agents lipopolysaccharide and CpG DNA in order to collect a concentrated sample of IL12R- β2. These samples will be analyzed for the presence of C-mannosyltryptophan residue

Subject(s)

Cytokines -- Research.

Glycosylation -- Research.

Proteins -- Analysis.

Cells -- Analysis.

Recommended Citation

Parsons, Michael Arland, "Isolation and Identification of Hsc70 Conjugates in RAW264.7 Murine Macrophage-Like Cells" (2019). Theses, Dissertations and Capstones. 1269.
https://mds.marshall.edu/etd/1269