Date of Award
2026
Degree Name
Pharmaceutical Sciences
College
School of Pharmacy
Type of Degree
M.S.
Document Type
Thesis
First Advisor
Dr. Cynthia Jones
Second Advisor
Dr. Eric Blough
Third Advisor
Dr. Timothy Long
Abstract
Chronic wounds, particularly in diabetic patients, remain a significant clinical challenge due to the complex interaction of persistent infection, chronic inflammation, and impaired cellular regeneration. Conventional drug therapies often fail to achieve effective treatment due to rapid drug degradation, poor targeting, and localized toxicity. This study explores a nanotechnology-based approach by developing and evaluating 5-FU loaded AgNP (AgNP–5FU) as a controlled drug delivery system. AgNP (AgNP) were synthesized and characterized using scanning electron microscopy (SEM), energy dispersive X-ray (EDX) analysis, particle size, and zeta potential analysis, while Fourier Transform Infrared Spectroscopy (FTIR) analysis suggested possible interaction between 5-FU and the nanoparticle surface. Biological evaluation using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on human dermal fibroblast cells (HDF-a) showed that AgNP-5FU formulations demonstrated reduced cytotoxicity in HDF-a cells compared to free 5-FU in vitro. These findings suggest that AgNP– 5FUs warrant further investigation in wound healing models as potential topical drug delivery platforms.
Subject(s)
Pharmaceutical chemistry.
Drugs -- Research.
Wound healing.
Nanoparticles.
Wounds and injuries -- Treatment.
Drug delivery systems.
Synthetic drugs.
Scanning electron microscopy.
Fourier transform infrared spectroscopy.
Biological evaluation.
Bromides.
Cells.
Fluorouracil.
Recommended Citation
Kasi, Kamal Nath, "Nanocomposite mediated delivery of 5-Fluorouracil using silver nanoparticles to promote wound healing" (2026). Theses, Dissertations and Capstones. 2058.
https://mds.marshall.edu/etd/2058
