Spherical Invasion Assay: A Novel Method to Measure Invasion of Cancer Cells

Authors

Stephen D. Richbart, Marshall University
Justin Merritt, Marshall UniversityFollow
Emily G. Moles, Marshall University
Kathleen C. Brown, Marshall University
Adeoluwa A. Adeluola, Marshall UniversityFollow
Paul T. Finch, Marshall UniversityFollow
Joshua A. Hess, Marshall UniversityFollow
Maria T. Tirona, Marshall University
Sarah Miles, Marshall UniversityFollow
Monica Valentovic, Marshall UniversityFollow
Piyali Dasgupta, Marshall UniversityFollow

Document Type

Article

Publication Date

2-2022

Abstract

The invasion of tumor cells into the neighboring blood vessels and lymph nodes is a vital step for distant metastasis. Traditionally, the invasive activity of growth factors (or the anti-invasive activity of drugs) is measured with the Boyden chamber assay. However, this assay has a few disadvantages like poor physiological relevance of transwell inserts and an inability to control chemokine gradients. The Boyden chamber assay is one of the most prevalent methods to measure the invasion of cancer cells. It would be advantageous to develop another assay that could validate the results of the Boyden chamber assay. With this in mind, our laboratory developed the spherical invasion assay (SIA) to measure the pro-invasive activity of human cancer cells. The SIA also circumvents some of the drawbacks of the Boyden chamber assay. The present manuscript measures the anti-invasive activity of the Src kinase inhibitor PP2 in A549 human non-small cell lung carcinoma (NSCLC) cells using the SIA. The SIA protocol is comprised of two steps. In the first step, A549 human NSCLC cells (treated or not with PP2) were mixed with Matrigel and seeded in the middle of an eight-well chamber slide. After 24 h, a second layer of Matrigel was overlaid over the first layer. Over the course of the next 24 h, the A549 cells invade from the primary to the secondary Matrigel layers. Subsequently, the cells are visualized by phase-contrast microscopy and the images obtained are quantified using ImageJ to calculate the anti-invasive activity of PP2 in A549 cells. The results of the SIA correlate well with Boyden chamber assays. The SIA may be adapted for multiple experimental designs, such as drug screening (to combat invasion and metastasis), measuring the pro-invasive activity of growth factors, and elucidating the signaling pathways underlying the pro-invasive/anti-invasive activity of biological modifiers.

Comments

Copyright © 2022 The Authors; exclusive licensee Bio-protocol LLC.

Recommended Citation

Richbart, S. D. et al. (2022). Spherical Invasion Assay: A Novel Method to Measure Invasion of Cancer Cells. Bio protocol 12(04): e4320. DOI: 10.21769/BioProtoc.4320.